• May 28, 2026 Od Units Absorbance lank" or "zero" button on the spectrophotometer. This sets the baseline absorbance to zero. 5. Remove the blank cuvette. 6. Fill a cuvette with the sample solution. 7. Place the sample cuvette in the spectrophotometer and measure the absorbance. 5. Dealing with Sc By Danielle Smith-Luettgen